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mtb strain h37rv atcc 27294  (ATCC)


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    ATCC mtb strain h37rv atcc 27294
    The organs were harvested from mice that were sacrificed 1 and 5 hours after they have received daily doses of these antibiotics for seven days. H&E stains of the lungs are also shown. The Kramnik mice were aerosol challenged with Mtb <t>H37Rv</t> 14 weeks prior to the initiation of antibiotic therapy.
    Mtb Strain H37rv Atcc 27294, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 2908 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mtb+h37rv/Mycobacterium+tuberculosis%3B+subsp%2E+tuberculosis/pmc12890097-74-0-3
    Average 98 stars, based on 2908 article reviews
    mtb strain h37rv atcc 27294 - by Bioz Stars, 2026-09
    98/100 stars

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    1) Product Images from "Identifying optimal combination regimens for therapy of Mycobacterium tuberculosis with an algorithmic approach: prospective predictions and validations"

    Article Title: Identifying optimal combination regimens for therapy of Mycobacterium tuberculosis with an algorithmic approach: prospective predictions and validations

    Journal: PLOS One

    doi: 10.1371/journal.pone.0324206

    The organs were harvested from mice that were sacrificed 1 and 5 hours after they have received daily doses of these antibiotics for seven days. H&E stains of the lungs are also shown. The Kramnik mice were aerosol challenged with Mtb H37Rv 14 weeks prior to the initiation of antibiotic therapy.
    Figure Legend Snippet: The organs were harvested from mice that were sacrificed 1 and 5 hours after they have received daily doses of these antibiotics for seven days. H&E stains of the lungs are also shown. The Kramnik mice were aerosol challenged with Mtb H37Rv 14 weeks prior to the initiation of antibiotic therapy.

    Techniques Used: Aerosol

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    Mutagenesis:

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    Article Snippet: Middlebrook 7H9/ADC/Tween 80 (ADC) consisted of 4.7 g/L Middlebrook 7H9 base, 5 g/L albumin fraction V, 2 g/L glucose, 0.2 % glycerol, 0.81 g/L NaCl, and 0.05 % Tween 80. .. For MIC testing, Mtb H37Rv (ATCC 27294), the Mtb cydC::aph mutant as well as the clinical strains of Mtb were grown in the corresponding media up to an OD650nm of 0.2–0.4 and diluted 1000-fold in the respective growth medium. ..

    other:

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    Article Snippet: Mycobacterium tuberculosis (Mtb) forms cellulose-enriched biofilms.. These biofilms play a crucial role in the tuberculosis pathogenesis and drug tolerance exhibited by Mtb in vivo.. However, the biophysical characteristics of the cellulose remain poorly explored.

    Cell Culture:

    Article Title: Zinc pyrithione impairs iron-sulfur cluster biogenesis in Mycobacterium tuberculosis.
    Article Snippet: .. Mtb H37Rv (ATCC 27294) and clinical isolates were cultured in Middlebrook 7H9 medium supplemented with 0.05% Tween 80 and 10% OADC (Oleic Albumin Dextrose Catalase) at 37°C with 5% CO2. ..

    Article Title: Zinc pyrithione impairs iron-sulfur cluster biogenesis in Mycobacterium tuberculosis
    Article Snippet: .. Mtb H37Rv (ATCC 27294) and clinical isolates were cultured in Middlebrook 7H9 medium supplemented with 0.05% Tween 80 and 10% OADC (Oleic Albumin Dextrose Catalase) at 37°C with 5% CO 2 . ..

    Injection:

    Article Title: Targeting Mycobacterium tuberculosis GAPDH elicits potent bactericidal responses by dysregulating enzyme activity, redox dynamics and iron acquisition.
    Article Snippet: Please cite this article as: Z. Gani, M.N.. Ahmad, A. Sindhu, A. Kumar, A. Kumari, M. Imran, P. Malik, A. Dhiman, V.K.. Yadav, G.L.



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    mtb strain h37rv atcc 27294  (ATCC)
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    The organs were harvested from mice that were sacrificed 1 and 5 hours after they have received daily doses of these antibiotics for seven days. H&E stains of the lungs are also shown. The Kramnik mice were aerosol challenged with Mtb <t>H37Rv</t> 14 weeks prior to the initiation of antibiotic therapy.
    Mtb Strain H37rv Atcc 27294, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    strains mtb h37rv  (ATCC)
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    The organs were harvested from mice that were sacrificed 1 and 5 hours after they have received daily doses of these antibiotics for seven days. H&E stains of the lungs are also shown. The Kramnik mice were aerosol challenged with Mtb <t>H37Rv</t> 14 weeks prior to the initiation of antibiotic therapy.
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    The organs were harvested from mice that were sacrificed 1 and 5 hours after they have received daily doses of these antibiotics for seven days. H&E stains of the lungs are also shown. The Kramnik mice were aerosol challenged with Mtb <t>H37Rv</t> 14 weeks prior to the initiation of antibiotic therapy.
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    mtb h37rv 398  (ATCC)
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    The organs were harvested from mice that were sacrificed 1 and 5 hours after they have received daily doses of these antibiotics for seven days. H&E stains of the lungs are also shown. The Kramnik mice were aerosol challenged with Mtb <t>H37Rv</t> 14 weeks prior to the initiation of antibiotic therapy.
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    The organs were harvested from mice that were sacrificed 1 and 5 hours after they have received daily doses of these antibiotics for seven days. H&E stains of the lungs are also shown. The Kramnik mice were aerosol challenged with Mtb <t>H37Rv</t> 14 weeks prior to the initiation of antibiotic therapy.
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    mtb h37rv reference strain  (ATCC)
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    Schematic of the experimental workflow for method validation using <t>H37Rv-spiked</t> dust samples
    Mtb H37rv Reference Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The organs were harvested from mice that were sacrificed 1 and 5 hours after they have received daily doses of these antibiotics for seven days. H&E stains of the lungs are also shown. The Kramnik mice were aerosol challenged with Mtb H37Rv 14 weeks prior to the initiation of antibiotic therapy.

    Journal: PLOS One

    Article Title: Identifying optimal combination regimens for therapy of Mycobacterium tuberculosis with an algorithmic approach: prospective predictions and validations

    doi: 10.1371/journal.pone.0324206

    Figure Lengend Snippet: The organs were harvested from mice that were sacrificed 1 and 5 hours after they have received daily doses of these antibiotics for seven days. H&E stains of the lungs are also shown. The Kramnik mice were aerosol challenged with Mtb H37Rv 14 weeks prior to the initiation of antibiotic therapy.

    Article Snippet: Mtb strain H37Rv ATCC 27294 was purchased from the American Type Culture Collection (Manassas, VA).

    Techniques: Aerosol

    Schematic of the experimental workflow for method validation using H37Rv-spiked dust samples

    Journal: BMC Infectious Diseases

    Article Title: Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust

    doi: 10.1186/s12879-025-12332-0

    Figure Lengend Snippet: Schematic of the experimental workflow for method validation using H37Rv-spiked dust samples

    Article Snippet: The MTB H37Rv reference strain (ATCC27294) was obtained from the American Type Culture Collection (ATCC, USA).

    Techniques: Biomarker Discovery

    Determination of optimal Percoll density gradient for H37Rv separation from dust. ( A , D) Photographs of discontinuous ( A ) 5-layer and ( D ) 3-layer Percoll density gradients. Methylene blue was added to select layers during gradient preparation for visual clarity. The distinct interphases after centrifugation are formed by intrinsic density differences. Pure H37Rv (acid-fast stained, red bacilli) and unprocessed dust particles were layered. The pink background in the MTB sample tubes originates from the suspension medium of the stained bacilli; the actual localization of MTB is indicated by the turbid, cloudy bands at the density interfaces. ( B , E ) Quantitative comparison of H37Rv DNA recovery efficiency across gradient fractions, as determined by qPCR. Data are presented as mean ± SD ( n = 3). Statistical significance was analyzed by one-way ANOVA for multi-group comparisons and unpaired t-test for pairwise comparisons (*** p < 0.001, **** p < 0.0001). ( C ) Representative microscopic fields (1000× magnification) of acid-fast stained bacilli recovered from each layer of the 5-layer gradient (scale bar: 100 μm)

    Journal: BMC Infectious Diseases

    Article Title: Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust

    doi: 10.1186/s12879-025-12332-0

    Figure Lengend Snippet: Determination of optimal Percoll density gradient for H37Rv separation from dust. ( A , D) Photographs of discontinuous ( A ) 5-layer and ( D ) 3-layer Percoll density gradients. Methylene blue was added to select layers during gradient preparation for visual clarity. The distinct interphases after centrifugation are formed by intrinsic density differences. Pure H37Rv (acid-fast stained, red bacilli) and unprocessed dust particles were layered. The pink background in the MTB sample tubes originates from the suspension medium of the stained bacilli; the actual localization of MTB is indicated by the turbid, cloudy bands at the density interfaces. ( B , E ) Quantitative comparison of H37Rv DNA recovery efficiency across gradient fractions, as determined by qPCR. Data are presented as mean ± SD ( n = 3). Statistical significance was analyzed by one-way ANOVA for multi-group comparisons and unpaired t-test for pairwise comparisons (*** p < 0.001, **** p < 0.0001). ( C ) Representative microscopic fields (1000× magnification) of acid-fast stained bacilli recovered from each layer of the 5-layer gradient (scale bar: 100 μm)

    Article Snippet: The MTB H37Rv reference strain (ATCC27294) was obtained from the American Type Culture Collection (ATCC, USA).

    Techniques: Centrifugation, Staining, Suspension, Comparison

    Viability assessment of H37Rv recovered via Percoll separation. ( A-E ) Macroscopic appearance of H37Rv colonies on LJ medium after 4 weeks of incubation. Left panels: Recovery standard controls (direct inoculation of pure H37Rv suspensions without dust). Right panels: Colonies recovered via Percoll separation from H37Rv-spiked dust samples. Initial spiking concentrations: ( A ) 10⁰, ( B ) 10¹, ( C ) 10², ( D ) 10³, ( E ) 10⁴ CFU/mL. ( F ) Quantitative comparison of viable MTB concentrations (CFU/mL) between Percoll-separated samples (from dust matrix) and direct inoculation controls (pure H37Rv). Colony counts were converted to CFU/mL for analysis. Bars represent mean ± SD ( n = 3). Statistical significance was determined by a paired t-test (ns, not significant; ** p < 0.01)

    Journal: BMC Infectious Diseases

    Article Title: Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust

    doi: 10.1186/s12879-025-12332-0

    Figure Lengend Snippet: Viability assessment of H37Rv recovered via Percoll separation. ( A-E ) Macroscopic appearance of H37Rv colonies on LJ medium after 4 weeks of incubation. Left panels: Recovery standard controls (direct inoculation of pure H37Rv suspensions without dust). Right panels: Colonies recovered via Percoll separation from H37Rv-spiked dust samples. Initial spiking concentrations: ( A ) 10⁰, ( B ) 10¹, ( C ) 10², ( D ) 10³, ( E ) 10⁴ CFU/mL. ( F ) Quantitative comparison of viable MTB concentrations (CFU/mL) between Percoll-separated samples (from dust matrix) and direct inoculation controls (pure H37Rv). Colony counts were converted to CFU/mL for analysis. Bars represent mean ± SD ( n = 3). Statistical significance was determined by a paired t-test (ns, not significant; ** p < 0.01)

    Article Snippet: The MTB H37Rv reference strain (ATCC27294) was obtained from the American Type Culture Collection (ATCC, USA).

    Techniques: Incubation, Comparison

    Detection of MTB in air-conditioning dust from TB hospital wards using qPCR. ( A–D ) Representative qPCR amplification curves for MTB DNA detected in 25 clinical dust samples. Dashed lines: samples processed by Percoll separation. Solid lines: samples processed by direct DNA extraction. ( E ) qPCR amplification profiles for positive controls (H37Rv genomic DNA, red lines) and negative controls (no-template, green lines). ( F ) Examples of abnormal amplification curves obtained from the direct extraction method. ( G ) Violin plots comparing MTB DNA copy numbers between Percoll-based separation and direct extraction methods. Horizontal lines within violins represent the median and interquartile range. Statistical significance was determined by the Mann-Whitney U test (**** p < 0.0001)

    Journal: BMC Infectious Diseases

    Article Title: Non-continuous Percoll density gradient: a method for purifying Mycobacterium tuberculosis from dust

    doi: 10.1186/s12879-025-12332-0

    Figure Lengend Snippet: Detection of MTB in air-conditioning dust from TB hospital wards using qPCR. ( A–D ) Representative qPCR amplification curves for MTB DNA detected in 25 clinical dust samples. Dashed lines: samples processed by Percoll separation. Solid lines: samples processed by direct DNA extraction. ( E ) qPCR amplification profiles for positive controls (H37Rv genomic DNA, red lines) and negative controls (no-template, green lines). ( F ) Examples of abnormal amplification curves obtained from the direct extraction method. ( G ) Violin plots comparing MTB DNA copy numbers between Percoll-based separation and direct extraction methods. Horizontal lines within violins represent the median and interquartile range. Statistical significance was determined by the Mann-Whitney U test (**** p < 0.0001)

    Article Snippet: The MTB H37Rv reference strain (ATCC27294) was obtained from the American Type Culture Collection (ATCC, USA).

    Techniques: Amplification, DNA Extraction, Extraction, MANN-WHITNEY